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Test distinguishes Parkinson’s disease from related ...
https://www.nih.gov/news-events/nih-research-matters/test-distinguishes-parkinsons-disease-related-condition
Researchers led by Dr. Claudio Soto from UTHealth in Houston used a test called protein misfolding cyclic amplification (PMCA) to detect small amounts of alpha-synuclein aggregates in cerebrospinal fluid (CSF). PMCA works by exposing regular alpha-synuclein protein to samples from patients that might contain misfolded alpha-synuclein.
Cyclic amplification of protein misfolding: application to ...
https://pubmed.ncbi.nlm.nih.gov/12127750/
1 Serono International SA, Geneva, Switzerland. [email protected]; PMID: 12127750 DOI: 10.1016/s0166-2236(02)02195-1 Abstract Diverse human disorders, including the majority of neurodegenerative diseases, are thought to arise from the misfolding and aggregation of protein. ... (PMCA), is conceptually analogous to DNA amplification by PCR ...
Test distinguishes Parkinson's disease from related ...
https://www.nia.nih.gov/news/test-distinguishes-parkinsons-disease-related-condition
Researchers led by Dr. Claudio Soto from UTHealth in Houston used a test called protein misfolding cyclic amplification (PMCA) to detect small amounts of alpha-synuclein aggregates in cerebrospinal fluid (CSF). PMCA works by exposing regular alpha-synuclein protein to samples from patients that might contain misfolded alpha-synuclein.
Protein Misfolding Cyclic Amplification - an overview ...
https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/protein-misfolding-cyclic-amplification
It was developed by Claudio Soto's group as a means of detecting small quantities of PrP Sc particles using iterative cycles of sonication and incubation in the presence of excess amounts of PrP C substrate. 60 PMCA has been optimized for the detection of prions in a range of biological samples including blood, 61,62 urine, 63 nasal brushings, 64 lymph nodes, 65 CSF 66 and brain. …
α-Syn-PMCA in cerebrospinal fluid has diagnostic …
https://jxym.amegroups.com/article/view/4279/5118
In a study recently published in JAMA Neurology, a team led by Claudio Soto at the University of Texas Houston Medical School, adapted protein misfolding cyclic amplification (PMCA) for highly sensitive detection of α-Syn aggregates (α-Syn-PMCA) and used it to distinguish CSF samples obtained from patients affected by PD from those of individuals affected by other neurologic …
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